Gram Staining 101

- Today I want to share with you what I did in the lab. What is gram staining? What is its purpose? It's a technique that is one of the first steps to help detect bacteria. Have you ever gone to the doctor because you got infected by bacteria? In order for professionals to know the basis of it, gram staining is a very useful tool for this study.

We have: Negative Gram and Positive Gram.

What is the difference?

Bacteria have peptidoglycan. Some of them have a thick layer of peptidoglycan. For the ones with thick layer is "positive gram." For the ones with thin layer is "negative gram." In the picture below, I only worked with positive gram. In the microscope, if you see the "little dots" in purple, that's because you found bacteria with positive gram. If you see pink, it's negative gram. But this coloring is due to dyes we have to use in order to be able to see what sort of gram that bacteria has.

Photo owned by Lifestyle Unstoppable.

If you love microbiology like me, and are interested to know more about it, this video below explains step by step. Enjoy!

https://www.youtube.com/watch?v=sxa46xKfIOY

- Written by Victoria P. Daros, Health Coach.

- Today I want to share with us what is happening in the laboratory. What is the tinction of Gram? What is your purpose? It is a technique that is one of the first steps to help detect bacteria. Have you ever gone to a doctor because you were infected with a bacteria? So that professionals can understand its basis, Gram tinction is a very useful tool for this study.

We have: Gram Negative and Gram Positive.

What is the difference?

Bacteria have peptidoglycan. Some of them have a thick layer of peptidoglycan. For those with a thick cover, it is "gram positive". For those with thin covers it is "negative gram". In the lower image, I only work with a positive goal. Under the microscope, if you see "points" in purple, it is because you find positive bacteria. If it is pink, it is negative. But this coloring is due to the dyes that we have to use to be able to see what type of grass this bacteria has.

Photo owned by Lifestyle Unstoppable.

Photo by: https://thisonevsthatone.com/gram-positive-vs-gram-negative/

If you love microbiology like you and are interested in knowing more about it, this video below explains it step by step. ¡Distructate!

https://www.youtube.com/watch?v=sxa46xKfIOY

-Written by Victoria P. Daros, Health Coach.

- Today I want to share with you what I did in the laboratory. What is Gram stain? What is your purpose? It is a technique that is one of the first steps to help detect bacteria. Have you ever gone to the doctor because you were infected with bacteria? For professionals to know the basis of this, Gram staining is a very useful tool for this study.

We have: Gram negative and Gram positive.

What is the difference?

Bacteria have peptidoglycan. Some of them have a thick layer of peptidoglycan. For those with a thick layer, it is “gram positive”. For those with a thin layer, it is “gram negative”. In the photo below, I only worked with gram positive. Under the microscope, if you see the purple "dots", it is because you found gram positive bacteria. If you see pink, it's gram negative. But this coloring is due to dyes that we have to use to be able to see what type of gram this bacteria has.

Photo owned by Lifestyle Unstoppable.

Photo by: https://thisonevsthatone.com/gram-positive-vs-gram-negative/

If If you love microbiology like me, and are more interested in the subject, watch the video below that explains it step by step. Have a good time!

https://www.youtube.com/watch?v=sxa46xKfIOY

-Written by Victória P. Daros, Health Coach.